Nanomedicine and Nanoscience Technology: Open Access
Bismuth Oxide Nanoparticle: The Potential of Apoptotic and Genetic Damage on MDBK Cell Line
Abstract
Asawir Esamaldeen Ebrahim Mohamed, Ayla Celik* and Derya Yetkin
Nowadays, nanoparticles have been used in almost all areas of life thanks to advances in nano scale technology. Among the available nanoparticles, Bismuth oxide nanoparticle is widely used in many products in terms of its properties such as antibacterial, antitumor and cytotoxic activity and antifungal. In this study, we aimed to determine the in vitro genetic damage potential and apoptotic effects of bismuth oxide nanoparticle having 90-210 nm size (~191,2 nm) range in MDBK cell line cultures. In this study; single cell gel electrophoresis (comet) method and flow cytometry method were used to determine genotoxic and apoptotic effect. The MDBK (Madin Darby Bovine Kidney) cells were treated with bismuth oxide at three concentrations of 30 µg/ml, 60 µg/ml and 90 µg/ml. No significant difference was found between the negative control group for GDI and DCP parameter (p>0.05). When 30 µg/ml concentration was considered, there was a significant difference between negative control and other groups for early and late apoptotic, necrotic cell and number of live cells (p<0.05). Considering the 60 µg/ml and 90 µg/ml concentration, there was a statistically significant difference between the other groups in terms of early apoptotic, necrotic and live cell counts (p<0.05). There was a significant difference between positive control and negative control in terms of early and late apoptotic, necrotic cell and number of live cells (p<0.05).